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Human Phospho-IRF-3 (S386) and Total IRF-3 PharmaGenie ELISA Kit (SBRS1864)

SKU:
SBRS1864
Product Type:
ELISA Kit
Reactivity:
Human
Applications:
ELISA
ELISA Type:
Sandwich
  • Human Phospho-IRF-3 S386 and Total IRF-3 PharmaGenie ELISA Kit SBRS1864
  • Human Phospho-IRF-3 S386 and Total IRF-3 PharmaGenie ELISA Kit SBRS1864
€820
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Description

Human Phospho-IRF-3 (S386) and Total IRF-3 PharmaGenie ELISA Kit (SBRS1864)

Product SKU:SBRS1864
Size:96T
Application:This ELISA kit recognizes Human IRF-3 phosphorylated at site Serine-386 as well as total IRF-3.
Uniprot:Q14653
Gene ID:3661
Gene Names:IRF3
Synonyms:Interferon regulatory factor 3 (IRF-3)
Target Species:Human
Compatible Sample Types:Cell Lysates, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Colorimetric
Quantitative/Semi-Quantitative:Semi-Quantitative
Storage/Stability:Upon receipt, the kit should be stored at -20°C. Please use within 6 months from the date of shipment.
  1. Pre-Coated 96-well Strip Microplate
  2. Wash Buffer
  3. Anti-Phospho Antibody
  4. HRP-Conjugated Secondary Antibody
  5. Assay Diluent
  6. TMB One-Step Substrate
  7. Stop Solution
  8. Lysis Buffer
  9. Positive Control Sample

Other materials and equipment required:

The Assay Genie Human Phospho-IRF-3 (S386) and Total IRF-3 PharmaGenie ELISA Kit (SBRS1864) will require other equipment and materials to carry out the assay. Please see list below for further details.

  • Distilled or deionized water
  • 100 ml and 1 liter graduated cylinders
  • Tubes to prepare sample dilutions
  • Protease and Phosphatase inhibitors
  • Precision pipettes to deliver 2 ul to 1 ml volumes
  • Adjustable 1-25 ml pipettes for reagent preparation
  • Benchtop rocker or shaker
  • Microplate reader capable of measuring absorbance at 450 nm
  1. Prepare all reagents and samples as instructed in the manual.
  2. Add 100 ul of sample or positive control to each well.
  3. Incubate 2.5 h at RT or O/N at 4 °C.
  4. Add 100 ul of prepared primary antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 ul of prepared 1X HRP-Streptavidin to each well.
  7. Incubate 1 h at RT.
  8. Add 100 ul of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 ul of Stop Solution to each well.
  11. Read at 450 nm immediately.
Product ImageHT29 cells were treated or untreated with Calyculin A and analyzed using this phosphoELISA and Western Blot.
Product ImageHT29 cells were treated with Calyculin A. Solubilize cells at 4 x 107 cells/ml in Cell Lysate Buffer. Serial dilutions of lysates were analyzed in this ELISA.

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